4A5Q
Crystal structure of the chitinase Chi1 fitted into the 3D structure of the Yersinia entomophaga toxin complex
EM specimen
| Staining | Yes |
| Staining material | uranyl formate |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 300 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 17 |
| Software | Situs,EMAN,Xmipp |
| Symmetry | C5 |
