4A4Z

CRYSTAL STRUCTURE OF THE S. CEREVISIAE DEXH HELICASE SKI2 BOUND TO AMPPNP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-11-24
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	82.794, 118.551, 129.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.430 - 2.400
R-factor	0.2402
Rwork	0.238
R-free	0.27540
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.600
Number of reflections	50510
<I/σ(I)>	17	3.1
Completeness [%]	99.9	99.9
Redundancy	5.5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		3% (W/V) PEG3350, 0.1M HEPES PH 7.0, 5% (V/V) ETHYLENE GLYCOL