4A4Y
Structure of the Cytosolic Domain of the Shigella T3SS component MxiG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-16 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 94.790, 94.790, 31.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.050 - 1.570 |
R-factor | 0.17724 |
Rwork | 0.176 |
R-free | 0.20414 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STRUCTURE OF SE-MET DERIVATIVE |
RMSD bond length | 0.028 |
RMSD bond angle | 2.527 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.040 | 1.610 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.050 | 1.100 |
Number of reflections | 23028 | |
<I/σ(I)> | 23.13 | 2.02 |
Completeness [%] | 99.8 | 98.1 |
Redundancy | 11 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | PROTEIN WAS CRYSTALLIZED FROM 0.085 M HEPES SODIUM PH 7.5, 1.7% POLYETHYLENE GLYCOL 400, 1.7 M AMMONIUM SULFATE, 15% GLYCEROL. |