4A4K
Crystal structure of the S. cerevisiae Ski2 insertion domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 230.463, 123.139, 153.201 |
| Unit cell angles | 90.00, 131.05, 90.00 |
Refinement procedure
| Resolution | 57.159 - 3.250 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.25650 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.354 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 50807 | |
| <I/σ(I)> | 8.4 | 1.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 3.5 M SODIUM FORMATE, 0.1 M MES PH 6.5 |
