4A4J
Crosstalk between Cu(I) and Zn(II) homeostasis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 22.780, 58.040, 24.780 |
| Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
| Resolution | 29.020 - 1.250 |
| R-factor | 0.14476 |
| Rwork | 0.143 |
| R-free | 0.17988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.840 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 15818 | |
| <I/σ(I)> | 13.6 | 4.2 |
| Completeness [%] | 96.2 | 87.5 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM HEPES PH 7.5 10% PEG 4000 5% ISOPROPANOL |
