4A4D
Crystal structure of the N-terminal domain of the Human DEAD-BOX RNA helicase DDX5 (P68)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-25 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.180, 73.801, 104.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.640 - 2.700 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jgn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MrBUMP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.080 | 0.480 |
Number of reflections | 7229 | |
<I/σ(I)> | 19.6 | 2.7 |
Completeness [%] | 98.9 | 99.9 |
Redundancy | 5.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 2% (V/V) TACSIMATE PH 4.0, 0.1 M BIS-TRIS PH 6.5, AND 20% (W/V) POLYETHYLENE GLYCOL 3350 |