4A3X
Structure of the N-terminal domain of the Epa1 adhesin (Epa1-Np) from the pathogenic yeast Candida glabrata, in complex with calcium and lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.481, 105.453, 69.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.020 - 1.650 |
| R-factor | 0.17358 |
| Rwork | 0.172 |
| R-free | 0.20969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjp |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.226 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES (MOLREP) |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 33625 | |
| <I/σ(I)> | 20 | 3.2 |
| Completeness [%] | 99.1 | 87.7 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 50 MM TRIS-HCL, 200 MM NACL, 10 MM CACL2, 20 MM LACTOSE, 100 MM HEPES PH 7.5, 22.5% PEG8000. |
