4A3R
Crystal structure of Enolase from Bacillus subtilis.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-30 |
Detector | ADSC CCD |
Spacegroup name | P 4 |
Unit cell lengths | 187.074, 187.074, 56.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.200 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.21770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iyx |
RMSD bond length | 0.008 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.090 | 0.520 |
Number of reflections | 96029 | |
<I/σ(I)> | 9.9 | 1.9 |
Completeness [%] | 96.2 | 90.8 |
Redundancy | 2.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.0 M TRI-SODIUM CITRATE 0.1 M, NA CACODYLATE PH 6.5 . |