4A3P
Structure of USP15 DUSP-UBL deletion mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.950, 44.250, 56.150 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 27.200 - 1.400 |
| R-factor | 0.15652 |
| Rwork | 0.153 |
| R-free | 0.22126 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pv1 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.891 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.330 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.030 | 0.200 |
| Number of reflections | 39193 | |
| <I/σ(I)> | 13.4 | 3.9 |
| Completeness [%] | 92.8 | 95 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.9 M (NH4)2SO4, 100 MM HEPES PH 7.0, 200 MM KI. |
