4A3O

Crystal structure of the USP15 DUSP-UBL monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 31
Unit cell lengths	100.419, 100.419, 71.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.324 - 2.200
R-factor	0.2023
Rwork	0.201
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pv1
RMSD bond length	0.008
RMSD bond angle	1.001
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.030	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.420
Number of reflections	61255
<I/σ(I)>	8	2.3
Completeness [%]	99.0	96.5
Redundancy	2.9	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		1.9 M (NH4)2SO4, 100 MM HEPES PH 7.0, 200 MM KI