4A3N

Crystal Structure of HMG-BOX of Human SOX17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	C 1 2 1
Unit cell lengths	94.281, 22.525, 66.668
Unit cell angles	90.00, 129.09, 90.00

Refinement procedure

Resolution	23.660 - 2.400
R-factor	0.202
Rwork	0.202
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f27
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.080	0.190
Number of reflections	4389
<I/σ(I)>	22.9	23.4
Completeness [%]	98.0	100
Redundancy	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1