4A3M
RNA Polymerase II initial transcribing complex with a 4nt DNA-RNA hybrid and soaked with AMPCPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 222.270, 392.990, 282.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.650 - 3.900 |
R-factor | 0.1544 |
Rwork | 0.154 |
R-free | 0.19010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.000 | 3.900 |
High resolution limit [Å] | 3.900 | 3.800 |
Rmerge | 0.870 | |
Number of reflections | 111231 | |
<I/σ(I)> | 6.1 | |
Completeness [%] | 90.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7 |