4A3C
RNA Polymerase II initial transcribing complex with a 5nt DNA-RNA hybrid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 221.410, 393.120, 282.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.850 - 3.500 |
R-factor | 0.1585 |
Rwork | 0.158 |
R-free | 0.18420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.340 |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.500 |
Rmerge | 0.890 |
Number of reflections | 154318 |
<I/σ(I)> | 8.1 |
Completeness [%] | 99.0 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7 |