4A3B

RNA Polymerase II initial transcribing complex with a 4nt DNA-RNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	222.090, 392.970, 281.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.890 - 3.500
R-factor	0.1662
Rwork	0.166
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.330
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Rmerge	0.180
Number of reflections	152991
<I/σ(I)>	6.3
Completeness [%]	95.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		pH 7