4A35
Crystal structure of human Mitochondrial enolase superfamily member 1 (ENOSF1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-25 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97625, 0.9762 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.770, 84.770, 316.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 1.740 |
| R-factor | 0.1535 |
| Rwork | 0.152 |
| R-free | 0.17560 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 1.840 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.040 | 0.790 |
| Number of reflections | 69165 | |
| <I/σ(I)> | 12.6 | 2.3 |
| Completeness [%] | 99.0 | 93.9 |
| Redundancy | 10.4 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 3350, 0.1 M BIS-TRIS 5.5 |
