4A2Q
Structure of duck RIG-I tandem CARDs and helicase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 314.970, 134.490, 102.260 |
| Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
| Resolution | 157.370 - 3.400 |
| R-factor | 0.2407 |
| Rwork | 0.239 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a2p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.111 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.110 | 0.870 |
| Number of reflections | 57366 | |
| <I/σ(I)> | 15.5 | 2.1 |
| Completeness [%] | 97.6 | 97.6 |
| Redundancy | 7.27 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12 MG/ML PROTEIN AND 0.2 M TRI-SODIUM CITRATE, 17% PEG 3350. |
