4A2P
Structure of duck RIG-I helicase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-30 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 192.130, 192.130, 48.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.590 - 3.000 |
| R-factor | 0.21511 |
| Rwork | 0.211 |
| R-free | 0.29652 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.956 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 0.710 |
| Number of reflections | 13392 | |
| <I/σ(I)> | 16 | 1.8 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 3.79 | 3.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12 MG PER ML PROTEIN AND 0.1 M NACL, 0.1M M TRI-SODIUM CITRATE PH 5.6, 12% PEG 4000. |
