4A2C
Crystal structure of galactitol-1-phosphate dehydrogenase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-18 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.720, 77.140, 107.510 |
Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
Resolution | 28.952 - 1.870 |
R-factor | 0.2158 |
Rwork | 0.213 |
R-free | 0.27290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dq4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.127 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 1.970 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.090 | 0.530 |
Number of reflections | 56481 | |
<I/σ(I)> | 9.2 | 2.5 |
Completeness [%] | 95.9 | 95.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | PH 6.5 |