4A2C
Crystal structure of galactitol-1-phosphate dehydrogenase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.720, 77.140, 107.510 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 28.952 - 1.870 |
| R-factor | 0.2158 |
| Rwork | 0.213 |
| R-free | 0.27290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dq4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.090 | 0.530 |
| Number of reflections | 56481 | |
| <I/σ(I)> | 9.2 | 2.5 |
| Completeness [%] | 95.9 | 95.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PH 6.5 |
