4A22
Structure of Mycobacterium tuberculosis fructose 1,6-bisphosphate aldolase bound to N-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 335.386, 42.977, 102.601 |
Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
Resolution | 45.415 - 1.900 |
R-factor | 0.1825 |
Rwork | 0.179 |
R-free | 0.22060 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.188 |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.900 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.080 |
Number of reflections | 114281 |
<I/σ(I)> | 10 |
Completeness [%] | 99.5 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 8000, 1.8 M LI2SO4, 50 MM TRIS/HOAC PH 7.5. |