4A1R
The Structure of Serratia marcescens Lip, a membrane bound component of the Type VI Secretion System.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 86 |
| Detector technology | CCD |
| Collection date | 2010-02-13 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.730, 77.750, 54.490 |
| Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
| Resolution | 39.820 - 1.920 |
| R-factor | 0.22392 |
| Rwork | 0.220 |
| R-free | 0.29158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL FROM SAD EXPERIMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.050 | 0.440 |
| Number of reflections | 43478 | |
| <I/σ(I)> | 20.6 | 3.7 |
| Completeness [%] | 98.2 | 95.8 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15 % PEG 3350, 200 MM NACL. |
