4A15
Crystal structure of an XPD DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 92 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 79.060, 79.060, 175.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.530 - 2.200 |
| R-factor | 0.19524 |
| Rwork | 0.192 |
| R-free | 0.25049 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vsf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.736 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.580 |
| Number of reflections | 30961 | |
| <I/σ(I)> | 9.4 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM MES (PH 6.5), 10 MM MGSO4, 5-30% 2-METHYL-2 4-PENTANEDIOL |
