4A12
Structure of the global transcription regulator FapR from Staphylococcus aureus in complex with DNA operator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-17 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 114.690, 249.940, 179.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.750 - 3.150 |
R-factor | 0.187 |
Rwork | 0.186 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a0x |
RMSD bond length | 0.007 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 3.320 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.100 | 0.570 |
Number of reflections | 44060 | |
<I/σ(I)> | 24.3 | 2.4 |
Completeness [%] | 98.2 | 93.7 |
Redundancy | 16 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.2 M SODIUM FORMATE |