4A12
Structure of the global transcription regulator FapR from Staphylococcus aureus in complex with DNA operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.690, 249.940, 179.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.750 - 3.150 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.100 | 0.570 |
| Number of reflections | 44060 | |
| <I/σ(I)> | 24.3 | 2.4 |
| Completeness [%] | 98.2 | 93.7 |
| Redundancy | 16 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2 M SODIUM FORMATE |
