4A10
Apo-structure of 2-octenoyl-CoA carboxylase reductase CinF from streptomyces sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.640, 85.220, 113.970 |
| Unit cell angles | 90.00, 107.45, 90.00 |
Refinement procedure
| Resolution | 48.480 - 2.250 |
| R-factor | 0.2124 |
| Rwork | 0.209 |
| R-free | 0.26867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3krt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 2.250 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.160 | 0.620 |
| Number of reflections | 107076 | |
| <I/σ(I)> | 8.78 | 3.7 |
| Completeness [%] | 98.6 | 98.3 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M TRIS PH7, 20% GLYCEROL, 12% PEG8000 |
