4A02
X-ray crystallographic structure of EfCBM33A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-08 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 32 |
Unit cell lengths | 52.109, 52.109, 59.653 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.900 - 0.950 |
R-factor | 0.0964 |
R-free | 0.11580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bem |
RMSD bond length | 0.017 |
RMSD bond angle | 0.032 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.900 | 1.000 |
High resolution limit [Å] | 0.950 | 0.950 |
Number of reflections | 112000 | |
<I/σ(I)> | 12.1 | 3.4 |
Completeness [%] | 96.0 | 86.6 |
Redundancy | 2.6 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 1.0 M K/NA TARTRATE, 0.1 M IMIDAZOLE PH 8.0 AND 0.2 M NACL. |