4ZY9
X-ray crystal structure of selenomethionine-labelled V110M mutant of chitosan-binding module 1 derived from chitosanase/glucanase from Paenibacillus sp. IK-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-11-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97865 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.233, 65.329, 69.887 |
| Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
| Resolution | 47.550 - 1.200 |
| R-factor | 0.20934 |
| Rwork | 0.208 |
| R-free | 0.24921 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.466 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.222 | |
| Number of reflections | 78378 | |
| <I/σ(I)> | 28.4 | 4.5 |
| Completeness [%] | 99.8 | 97 |
| Redundancy | 6.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium fluoride, PEG 3350 |
