4ZY8
K. lactis Lst4 longin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 120.703, 120.703, 93.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.290 - 2.140 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.25220 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.290 | 2.216 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.066 | 0.632 |
| Number of reflections | 38603 | |
| <I/σ(I)> | 20.4 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 292 | Sodium chloride, Butan-1-ol, Bis-Tris |
