4ZXQ
P22 Tail Needle Gp26 1-140 crystallized at pH 3.9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2008-05-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.952, 94.095, 97.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.862 - 2.750 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2poh |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 29142 | |
| <I/σ(I)> | 20.4 | 4.3 |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.9 | 293 | 20% PEG 8000, 0.05 M Potassium phosphate monobasic pH 3.9 |
