4ZWN
Crystal Structure of a Soluble Variant of the Monoglyceride Lipase from Saccharomyces Cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.196, 108.566, 167.667 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.270 - 2.491 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22400 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.771 |
Data scaling software | Aimless (0.1.29) |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.270 | 45.270 | 2.570 |
High resolution limit [Å] | 2.490 | 9.960 | 2.490 |
Rmerge | 0.198 | 0.061 | 1.498 |
Rpim | 0.070 | 0.022 | 0.536 |
Total number of observations | 439569 | 6519 | 35355 |
Number of reflections | 49134 | ||
<I/σ(I)> | 10.5 | 30.8 | 1.7 |
Completeness [%] | 98.4 | 94.6 | 91.6 |
Redundancy | 8.9 | 8 | 8.5 |
CC(1/2) | 0.991 | 0.996 | 0.490 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 293.15 | 0.1 M Bicine/Trizma base pH 8.7, 10% w/v PEG 20 000, 20% v/v PEG MME 550 and 0.03 M sodium nitrate, 0.03 M disodium hydrogen phosphate, 0.03 M ammonium sulphate. Microseeding |