4ZW9
Crystal structure of human GLUT3 bound to D-glucose in the outward-occluded conformation at 1.5 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.338, 118.132, 51.341 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 30.226 - 1.502 |
| R-factor | 0.1843 |
| Rwork | 0.184 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pyp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.132 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.086 | 0.578 |
| Number of reflections | 88120 | |
| <I/σ(I)> | 12.7 | 3.6 |
| Completeness [%] | 98.8 | 91.8 |
| Redundancy | 18 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.8 | 293 | 28%(v/v) PEG400, 0.1M HEPES, 50 mM ammonium citrate |
