4ZVG
Crystal structure of GGDEF domain of the E. coli DosC - form III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.780, 58.930, 113.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.610 - 2.200 |
| R-factor | 0.2098 |
| Rwork | 0.208 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zve |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.783 |
| Number of reflections | 18580 | |
| <I/σ(I)> | 11.36 | 2.54 |
| Completeness [%] | 97.9 | 95.3 |
| Redundancy | 5.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M potassium sodium tartrate, 22% (w/v) PEG 3350 |
