4ZVB
Crystal structure of globin domain of the E. coli DosC - form II (ferrous)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7345 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.960, 58.270, 59.400 |
| Unit cell angles | 79.52, 75.69, 75.29 |
Refinement procedure
| Resolution | 45.341 - 2.400 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zva |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.698 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.752 |
| Number of reflections | 22142 | |
| <I/σ(I)> | 12.36 | 2.64 |
| Completeness [%] | 94.6 | 88.3 |
| Redundancy | 10.2 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M sodium phosphate-citrate pH 4.2, 0.2 M sodium chloride, 16% (w/v) PEG 3000 |
