4ZV9
2.00 Angstrom resolution crystal structure of an uncharacterized protein from Escherichia coli O157:H7 str. Sakai
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.615, 96.341, 129.617 |
| Unit cell angles | 90.00, 98.22, 90.00 |
Refinement procedure
| Resolution | 38.790 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.362 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.550 |
| Number of reflections | 115793 | |
| <I/σ(I)> | 21.7 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 295 | Crystallization: The JCSG+ Suite (D12): 0.04 M Potasium phosphate, 16% (w/v) PEG 8000, 20% (v/v) glycerol Protein: 8.7 mg/ml in 10 mM Tris-HCl pH 8.3, 0.25 M NaCl, 5 mM BME Freezing: crystallization condition |
