4ZV2
An ancestral arginine-binding protein bound to glutamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.200, 61.300, 104.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.210 - 1.430 |
| R-factor | 0.18293 |
| Rwork | 0.182 |
| R-free | 0.20487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zv1 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.299 |
| Data reduction software | MOSFLM (7.0.9) |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | MOLREP (11.1.00) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.178 | 1.871 |
| Number of reflections | 43838 | |
| <I/σ(I)> | 9.2 | 1.5 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 12.7 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.2 M MgCl2, 0.1 M HEPES pH 7.5, 24% (w/v) PEG 3350, microseeds from crushed crystals produced using same precipitant |
