4ZV0

Structure of Tse6 in complex with Tsi6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	83.209, 83.209, 83.758
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.261 - 1.401
R-factor	0.1471
Rwork	0.146
R-free	0.16430
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	1.713
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.370	1.370
Rmerge	0.082	0.617
Number of reflections	69121
<I/σ(I)>	33.5
Completeness [%]	99.9	99.5
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% (w/v) PEG 3350, 0.2M ammonium iodide