4ZUT
Crystal structure of Equine MHC I(Eqca-N*00602) in complexed with equine infectious anaemia virus (EIAV) derived peptide Gag-GW12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97900 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.793, 69.793, 207.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.623 - 2.600 |
R-factor | 0.2235 |
Rwork | 0.221 |
R-free | 0.26510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q94 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.759 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.600 | 2.500 |
Rmerge | 0.084 | 0.587 |
Number of reflections | 16706 | |
<I/σ(I)> | 38.407 | 5.314 |
Completeness [%] | 99.2 | 99.8 |
Redundancy | 11.3 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.02 M magnesium chloride hexahydrare, 0.1 M HEPES, 22% w/v polyacrylic acid sodium salt 5,100 |