4ZUO
Crystal structure of acetylpolyamine amidohydrolase from Mycoplana ramosa in complex with a hydroxamate inhibitor
Replaces: 4K6QExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.069 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.998, 121.076, 64.417 |
| Unit cell angles | 90.00, 96.75, 90.00 |
Refinement procedure
| Resolution | 38.840 - 1.330 |
| R-factor | 0.1336 |
| Rwork | 0.133 |
| R-free | 0.14740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q9b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1833) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.054 | 0.272 |
| Number of reflections | 155090 | |
| <I/σ(I)> | 23.4 | 6.8 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 M NH4Cl, 20% (w/v) PEG 3,350 Additional 2 day soaking in precipitant solution supplemented with 2.5 mM inhibitor |
