4ZUJ
Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-04-21 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.089, 60.464, 38.262 |
| Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
| Resolution | 31.030 - 1.720 |
| R-factor | 0.1748 |
| Rwork | 0.173 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.996 |
| Data reduction software | XPREP |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.028 | 0.176 |
| Total number of observations | 58673 | |
| Number of reflections | 15097 | |
| <I/σ(I)> | 22.26 | 4.88 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.81 | 2.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
