4ZTZ

Structural basis for processivity and antiviral drug toxicity in human mitochondrial DNA replicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	217.600, 217.600, 165.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.500 - 3.442
R-factor	0.281
Rwork	0.280
R-free	0.32000
RMSD bond length	0.011
RMSD bond angle	2.119
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.500
High resolution limit [Å]	3.442
Number of reflections	48718
<I/σ(I)>	2
Completeness [%]	99.6
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	297	PEG8000, KCl, CaCl2