4ZTU

Structural basis for processivity and antiviral drug toxicity in human mitochondrial DNA replicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979,1.00
Spacegroup name	P 41 21 2
Unit cell lengths	217.575, 217.575, 168.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.670 - 3.299
Rwork	0.316
R-free	0.31640
RMSD bond length	0.005
RMSD bond angle	0.809
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.600
High resolution limit [Å]	3.299
Rmerge	0.080
Number of reflections	61142
<I/σ(I)>	2
Completeness [%]	99.6
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	297	PEG8000, KCl, CaCl2