4ZTT
Crystal structures of ferritin mutants reveal diferric-peroxo intermediates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 298.15 |
| Detector technology | CCD |
| Collection date | 2013-11-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 4 |
| Unit cell lengths | 128.816, 128.816, 172.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.660 - 1.830 |
| R-factor | 0.15813 |
| Rwork | 0.156 |
| R-free | 0.19191 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.061 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 154787 |
| <I/σ(I)> | 17 |
| Completeness [%] | 91.6 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | Lithium sulfate monohydrate, sodium citrate, ammonium sulfate |
