4ZTJ
Crystal Structure of the Prototype Foamy Virus Intasome with a 2-Pyridinone Aminal Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 158.960, 158.960, 124.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.830 - 2.670 |
R-factor | 0.1969 |
Rwork | 0.196 |
R-free | 0.22110 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.11.5) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.001 | 49.001 | 2.679 |
High resolution limit [Å] | 2.670 | 12.328 | 2.670 |
Rmerge | 0.093 | 0.063 | 0.972 |
Total number of observations | 610998 | 6061 | 5883 |
Number of reflections | 45839 | ||
<I/σ(I)> | 18.7 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13.3 | 11.1 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.25 M AMMONIUM SULFATE, 25% (V/V) GLYCEROL, 4.8% (V/V) 1,6-HEXANEDIOL, 50 MM MES-NAOH, 1MM EDTA, PH 6.5 |