4ZTG

Ebola virus nucleoprotein bound to VP35 chaperoning peptide P22121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 2 21 21
Unit cell lengths	33.349, 92.398, 133.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.800
R-factor	0.2625
Rwork	0.259
R-free	0.29330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.593
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.200	46.200	2.950
High resolution limit [Å]	2.800	8.840	2.800
Rmerge	0.084	0.033	1.042
Rpim	0.036	0.015	0.449
Total number of observations	68091	2132	9268
Number of reflections	10825
<I/σ(I)>	12.9	39.6	1.6
Completeness [%]	99.7	99	98.4
Redundancy	6.3	5.2	6.2
CC(1/2)	0.999	0.999	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	12% PEG 6000, 100 mM MES pH 5.0