4ZTF
Crystal Structure of the Prototype Foamy Virus Intasome with a 2-Pyridinone Aminal Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.450, 159.450, 124.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.858 - 2.700 |
R-factor | 0.1922 |
Rwork | 0.191 |
R-free | 0.21460 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (2.11.5) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.858 | 48.858 | 2.709 |
High resolution limit [Å] | 2.700 | 12.463 | 2.700 |
Rmerge | 0.107 | 0.062 | 0.977 |
Total number of observations | 489046 | 4997 | 4686 |
Number of reflections | 44469 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 11 | 9.5 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.25 M AMMONIUM SULFATE, 25% (V/V) GLYCEROL, 4.8% (V/V) 1,6-HEXANEDIOL, 50 MM MES-NAOH, 1MM EDTA, PH 6.5 |