4ZSN
70S-wild-type HigB toxin complex bound to a AAA lysine codon
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 213.446, 452.916, 608.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.920 - 3.600 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.920 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.216 | 0.828 |
| Number of reflections | 666396 | |
| <I/σ(I)> | 6.6 | 1.8 |
| Completeness [%] | 98.7 | 99.2 |
| Redundancy | 5.18 | 4.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN |
