4ZS6
Receptor binding domain and Fab complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-06 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.492, 64.461, 186.049 |
Unit cell angles | 90.00, 100.43, 90.00 |
Refinement procedure
Resolution | 36.953 - 3.166 |
R-factor | 0.2055 |
Rwork | 0.203 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l72 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.494 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.270 |
High resolution limit [Å] | 3.166 | 7.880 | 3.166 |
Rmerge | 0.170 | 0.085 | 0.876 |
Total number of observations | 156458 | ||
Number of reflections | 31146 | ||
<I/σ(I)> | 7.1 | ||
Completeness [%] | 95.1 | 90.1 | 96.7 |
Redundancy | 5 | 4.6 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M MES monohydrate (pH 6.5), 12% (w/v) Polyethylene glycol 20000 |