4ZS0
Human Aurora A catalytic domain bound to SB-6-OH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.880, 82.880, 171.392 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.400 - 3.000 |
| R-factor | 0.20475 |
| Rwork | 0.202 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.209 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.076 | 0.600 |
| Number of reflections | 7543 | |
| <I/σ(I)> | 18.5 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 4% v/v Tacsimate pH 8.0, 12% w/v PEG 3350 |
