4ZPX
Crystal structure of Lon ATPase domain from Thermococcus onnurineus NA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 40.620, 61.599, 76.321 |
Unit cell angles | 74.61, 87.11, 83.45 |
Refinement procedure
Resolution | 41.140 - 2.030 |
R-factor | 0.18444 |
Rwork | 0.182 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.856 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.030 |
Number of reflections | 42270 |
<I/σ(I)> | 23.03 |
Completeness [%] | 93.1 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | 0.2M Potassium chloride, 0.01M Magnesium acetate, 0.05M tri-sodium citrate dihydrate pH 4.5, 12% PEG 4000, 5% n-Dodecyl-beta-D-maltoside |