4ZPO
Crystal Structure of Protocadherin Gamma C5 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 108.637, 108.637, 96.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.064 - 2.900 |
| R-factor | 0.2216 |
| Rwork | 0.220 |
| R-free | 0.24720 |
| Structure solution method | MIR |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.514 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.064 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.360 |
| Number of reflections | 13086 | |
| <I/σ(I)> | 19.8 | 5.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.2 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 40% (v/v) MPD, 5% (w/v) PEG 8000, 100mM sodium cacodylate, pH 6 |
