4ZP4
Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.006, 76.463, 98.817 |
| Unit cell angles | 90.06, 90.19, 73.13 |
Refinement procedure
| Resolution | 49.408 - 2.355 |
| R-factor | 0.2032 |
| Rwork | 0.200 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 4F3L 3f1p & 4M4X |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.061 | 0.049 | 0.700 |
| Rmeas | 0.077 | 0.061 | 0.900 |
| Rpim | 0.046 | 0.036 | 0.557 |
| Total number of observations | 144295 | ||
| Number of reflections | 55780 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 98.4 | 95.9 | 97.4 |
| Redundancy | 2.6 | 2.5 | 2.5 |
| CC(1/2) | 0.992 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2% Tacsimate, pH 7.0, 6% PEG3350 |
