4ZP4

Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 1
Unit cell lengths	49.006, 76.463, 98.817
Unit cell angles	90.06, 90.19, 73.13

Refinement procedure

Resolution	49.408 - 2.355
R-factor	0.2032
Rwork	0.200
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4F3L 3f1p & 4M4X
RMSD bond length	0.011
RMSD bond angle	1.403
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.390
High resolution limit [Å]	2.350	6.370	2.350
Rmerge	0.061	0.049	0.700
Rmeas	0.077	0.061	0.900
Rpim	0.046	0.036	0.557
Total number of observations	144295
Number of reflections	55780
<I/σ(I)>	13
Completeness [%]	98.4	95.9	97.4
Redundancy	2.6	2.5	2.5
CC(1/2)		0.992	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	2% Tacsimate, pH 7.0, 6% PEG3350