4ZOR
The structure of the S37P MS2 viral capsid assembly.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | F 2 3 |
Unit cell lengths | 228.125, 228.125, 228.125 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.900 - 2.200 |
R-factor | 0.219 |
Rwork | 0.218 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ms2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.989 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (DEV_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.900 | 2.281 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.145 | 1.577 |
Number of reflections | 49435 | |
<I/σ(I)> | 7.7 | 1.02 |
Completeness [%] | 99.9 | 0.99 |
Redundancy | 5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 299 | 1.8M lithium sulphate, 100mM TRIS, pH 7.55, 20% glycerol (cryo) |